anti syp (Cell Signaling Technology Inc)
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Anti Syp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psd95/PSD95+Mouse+mAb/pmc12805289-48-12-16
Average 96 stars, based on 136 article reviews
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Enzyme-linked Immunosorbent Assay:Article Title: Effect and mechanism of compound Nujia honey paste on chronic unpredictable mild stress (CUMS) depression rats based on network pharmacology analysis. Article Snippet: Ethnopharmacological relevance: Compound Nujia honey paste (Nujia), a classic formulation from Traditional Uyghur Medicine, has been historically used for depression treatment and is listed in the Catalog of Ancient Classical Famous Formulas issued by the National Administration of Traditional Chinese Medicine and the National Medical Products Administration.. Clarifying its pharmacodynamic material basis is essential for understanding its efficacy, yet this remains incompletely characterized.. Aim of the study: This study aimed to systematically elucidate Nujia's antidepressant efficacy and mechanisms by combining chemical analysis, computational prediction, and experimental validation in a CUMS rat model, providing a comprehensive approach to understanding its action. Incubation:Article Title: Phlorotannin supplement mitigates cognitive impairment by enhancing synaptic plasticity in SAMP8 mice Article Snippet: .. Membranes were blocked with 5% bovine serum albumin in TBST and incubated overnight at 4 °C with primary antibodies against synapsin 1, Article Title: Effects of Short-Term Exposure to Polystyrene Nanoplastics on the Nervous System: Calcium Homeostasis, BDNF and Synaptic Plasticity. Article Snippet: Proteins were then separated using an SDS-PAGE gel preparation kit (Solarbio, P1200) for hierarchical separation, followed by membrane transfer. .. The membranes were blocked for 1 h at room temperature with 5% skim milk (Beyotime, Shanghai, China, P0216-1500 g) and incubated overnight at 4 ◦C with specific primary antibodies, including Cav3.1 (1:2000, NBP2-59322, Novus, Lone Tree, CO, USA), CaMKIIα (1:2000, 3357S, CST, Danvers, MA, USA), CREB (1:2000, 12208-1-AP, Proteintech, Rosemont, IL, USA), BDNF (1:1000, ab226843, abcam, Cambridge, UK), other:Article Title: A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex Article Snippet: Pan-γ-Pcdh N159/5 (Mouse, 1:500, NeuroMab 75−185, RRID:AB_2877195); Pan-γ-Pcdh-A N144/32 (Mouse, 1:500, NeuroMab 75−178 RRID:AB_2877459); γ-Pcdh-B2 N148/30 (Mouse, 1:500, NeuroMab 75−184, RRID:AB_2877457); γ-Pcdh-C5 (Mouse, 1:500, Invitrogen MA5−26922 RRID:AB_2724958); GAPDH (Mouse, 1:1000, Abcam 8245, RRID:AB_2107448); Article Title: A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex. Article Snippet: Pan-γ-Pcdh N159/5 (Mouse, 1:500, NeuroMab 75−185, RRID:AB_2877195); Pan-γ-Pcdh-A N144/32 (Mouse, 1:500, NeuroMab 75−178 RRID:AB_2877459); γ-Pcdh-B2 N148/30 (Mouse, 1:500, NeuroMab 75−184, RRID:AB_2877457); γ-Pcdh-C5 (Mouse, 1:500, Invitrogen MA5−26922 RRID:AB_2724958); GAPDH (Mouse, 1:1000, Abcam 8245, RRID:AB_2107448); Immunostaining:Article Title: Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology. Article Snippet: Human protein chip assay screening for Aβ-binding proteins A human proteome chip (HuProtTM v3.1, CDI Labs), printed on 75 mm × 25 mm SuperEpoxy 3 glass slides (ArrayIt Corp.) and containing over 20,000 purified full-length human recombinant proteins, was blocked with 2% BSA in PBST for 2 h at room temperature and incubated with Alexa Fluor 532-labeled Aβ (3 μg) at 4 °C for 8 h. After washing and rinsing, the array was dried and scanned using a microarray laser scanner. .. 6E10 (Biolegend, 803003); Aβ (β-Amyloid Rabbit mAb, ABclonal, A26342 for analyzing tissue samples); Kvβ2 (Origene, TA331456 for immunostaining; Neuromab, 75- 021 for Western blot and co-IP); MAP2 (Biolegend, 822501); Ankyrin G (Santa Cruz Biotechnology, sc-12719); EB1 (Santa Cruz Biotechnology, sc47704; ABclonal, A2614); Kv1.2 (ABclonal, A6295); c-Fos (Abcam, AB190289); NeuN (Merck, MAB377); VGLUT1 (ABclonal, A17188); Western Blot:Article Title: Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology. Article Snippet: Human protein chip assay screening for Aβ-binding proteins A human proteome chip (HuProtTM v3.1, CDI Labs), printed on 75 mm × 25 mm SuperEpoxy 3 glass slides (ArrayIt Corp.) and containing over 20,000 purified full-length human recombinant proteins, was blocked with 2% BSA in PBST for 2 h at room temperature and incubated with Alexa Fluor 532-labeled Aβ (3 μg) at 4 °C for 8 h. After washing and rinsing, the array was dried and scanned using a microarray laser scanner. .. 6E10 (Biolegend, 803003); Aβ (β-Amyloid Rabbit mAb, ABclonal, A26342 for analyzing tissue samples); Kvβ2 (Origene, TA331456 for immunostaining; Neuromab, 75- 021 for Western blot and co-IP); MAP2 (Biolegend, 822501); Ankyrin G (Santa Cruz Biotechnology, sc-12719); EB1 (Santa Cruz Biotechnology, sc47704; ABclonal, A2614); Kv1.2 (ABclonal, A6295); c-Fos (Abcam, AB190289); NeuN (Merck, MAB377); VGLUT1 (ABclonal, A17188); |
