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anti syp  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti syp
    Anti Syp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psd95/PSD95+Mouse+mAb/pmc12805289-48-12-16
    Average 96 stars, based on 136 article reviews
    anti syp - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effect and mechanism of compound Nujia honey paste on chronic unpredictable mild stress (CUMS) depression rats based on network pharmacology analysis.
    Article Snippet: Ethnopharmacological relevance: Compound Nujia honey paste (Nujia), a classic formulation from Traditional Uyghur Medicine, has been historically used for depression treatment and is listed in the Catalog of Ancient Classical Famous Formulas issued by the National Administration of Traditional Chinese Medicine and the National Medical Products Administration.. Clarifying its pharmacodynamic material basis is essential for understanding its efficacy, yet this remains incompletely characterized.. Aim of the study: This study aimed to systematically elucidate Nujia's antidepressant efficacy and mechanisms by combining chemical analysis, computational prediction, and experimental validation in a CUMS rat model, providing a comprehensive approach to understanding its action.

    Incubation:

    Article Title: Phlorotannin supplement mitigates cognitive impairment by enhancing synaptic plasticity in SAMP8 mice
    Article Snippet: .. Membranes were blocked with 5% bovine serum albumin in TBST and incubated overnight at 4 °C with primary antibodies against synapsin 1, PSD95, α-tubulin, NMDAR2B, p-Ca2+/calmodulin–dependent protein kinase II (CaMKII), CaMKII, p-cAMP response elementbinding protein (CREB), CREB, BDNF, p-extracellular signal–regulated kinases (ERK), and ERK (Cell Signaling Technology, Massachusetts, USA). .. Protein bands were detected using an enhanced chemiluminescence imaging system (LuminoGraph II EM, ATTO, Japan) and band intensities were quantified using ImageJ software.

    Article Title: Effects of Short-Term Exposure to Polystyrene Nanoplastics on the Nervous System: Calcium Homeostasis, BDNF and Synaptic Plasticity.
    Article Snippet: Proteins were then separated using an SDS-PAGE gel preparation kit (Solarbio, P1200) for hierarchical separation, followed by membrane transfer. .. The membranes were blocked for 1 h at room temperature with 5% skim milk (Beyotime, Shanghai, China, P0216-1500 g) and incubated overnight at 4 ◦C with specific primary antibodies, including Cav3.1 (1:2000, NBP2-59322, Novus, Lone Tree, CO, USA), CaMKIIα (1:2000, 3357S, CST, Danvers, MA, USA), CREB (1:2000, 12208-1-AP, Proteintech, Rosemont, IL, USA), BDNF (1:1000, ab226843, abcam, Cambridge, UK), PSD95 (1:1000, 2507S, CST), SYN (1:2000, 17785-1-AP, Proteintech), ProBDNF (1:1000, 28205-1-AP, Proteintech), and GAPDH (1:2000, UM4002, YouKang, Tianjin, China). .. The membrane was then incubated with secondary antibodies, sheep anti-rabbit IgG-HRP (1:4000, S0001, Affinity Biosciences, Jiangsu, China) and sheep anti-mouse IgG-HRP (1:4000, S0002, Affinity) for 1 h at room temperature.

    other:

    Article Title: A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex
    Article Snippet: Pan-γ-Pcdh N159/5 (Mouse, 1:500, NeuroMab 75−185, RRID:AB_2877195); Pan-γ-Pcdh-A N144/32 (Mouse, 1:500, NeuroMab 75−178 RRID:AB_2877459); γ-Pcdh-B2 N148/30 (Mouse, 1:500, NeuroMab 75−184, RRID:AB_2877457); γ-Pcdh-C5 (Mouse, 1:500, Invitrogen MA5−26922 RRID:AB_2724958); GAPDH (Mouse, 1:1000, Abcam 8245, RRID:AB_2107448); CC3 (Rabbit, 1:100, Cell Signaling 9661, RRID:AB_2341188); GFAP (Mouse, 1:500, Sigma-Aldrich G3893, RRID:AB_477010); NeuN (Mouse, 1:300, Millipore MAB377 RRID:AB_2298772); Cux1 (Rabbit, 1:100, Proteintech 11733–1-AP RRID:AB_2086995); Foxp2 (Rabbit, 1:500, Abcam 16046 RRID:AB_2107107); PSD95 (Mouse, 1:1000, Cell Signaling 36233 RRID:AB_2721262); Gephyrin (Rabbit, 1:1000, Cell Signaling 14304, RRID:AB_2798443); Synapsin (Rabbit, 1:1000, Cell Signaling 5297 RRID:AB_2616578).

    Article Title: A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex.
    Article Snippet: Pan-γ-Pcdh N159/5 (Mouse, 1:500, NeuroMab 75−185, RRID:AB_2877195); Pan-γ-Pcdh-A N144/32 (Mouse, 1:500, NeuroMab 75−178 RRID:AB_2877459); γ-Pcdh-B2 N148/30 (Mouse, 1:500, NeuroMab 75−184, RRID:AB_2877457); γ-Pcdh-C5 (Mouse, 1:500, Invitrogen MA5−26922 RRID:AB_2724958); GAPDH (Mouse, 1:1000, Abcam 8245, RRID:AB_2107448); CC3 (Rabbit, 1:100, Cell Signaling 9661, RRID:AB_2341188); GFAP (Mouse, 1:500, Sigma-Aldrich G3893, RRID:AB_477010); NeuN (Mouse, 1:300, Millipore MAB377 RRID:AB_2298772); Cux1 (Rabbit, 1:100, Proteintech 11733–1-AP RRID:AB_2086995); Foxp2 (Rabbit, 1:500, Abcam 16046 RRID:AB_2107107); PSD95 (Mouse, 1:1000, Cell Signaling 36233 RRID:AB_2721262); Gephyrin (Rabbit, 1:1000, Cell Signaling 14304, RRID:AB_2798443); Synapsin (Rabbit, 1:1000, Cell Signaling 5297 RRID:AB_2616578).

    Immunostaining:

    Article Title: Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology.
    Article Snippet: Human protein chip assay screening for Aβ-binding proteins A human proteome chip (HuProtTM v3.1, CDI Labs), printed on 75 mm × 25 mm SuperEpoxy 3 glass slides (ArrayIt Corp.) and containing over 20,000 purified full-length human recombinant proteins, was blocked with 2% BSA in PBST for 2 h at room temperature and incubated with Alexa Fluor 532-labeled Aβ (3 μg) at 4 °C for 8 h. After washing and rinsing, the array was dried and scanned using a microarray laser scanner. .. 6E10 (Biolegend, 803003); Aβ (β-Amyloid Rabbit mAb, ABclonal, A26342 for analyzing tissue samples); Kvβ2 (Origene, TA331456 for immunostaining; Neuromab, 75- 021 for Western blot and co-IP); MAP2 (Biolegend, 822501); Ankyrin G (Santa Cruz Biotechnology, sc-12719); EB1 (Santa Cruz Biotechnology, sc47704; ABclonal, A2614); Kv1.2 (ABclonal, A6295); c-Fos (Abcam, AB190289); NeuN (Merck, MAB377); VGLUT1 (ABclonal, A17188); PSD95 (Cell Signaling Technology, 3409); HA (Santa Cruz Biotechnology, sc7392); His (Cell Signaling Technology, 2365); V5 (Abcam, ab27671); FLAG (Sigma, F1804); RFP (MBL, PM005); GFP (Santa Cruz Biotechnology, sc9996); α-Tubulin (Santa Cruz Biotechnology, sc-23948); HRP-Streptavidin (Sigma, RABHRP3); normal mouse IgG (Santa Cruz Biotechnology, sc3877) and normal rabbit IgG (Cell Signaling Technology, 2729S) antibodies were used as controls. .. Intracellular proteins with A-scores exceeding 10,000 were then selected for data presentation (GENEON BIOTECH, Daejeon, Republic of Korea) [8].

    Western Blot:

    Article Title: Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology.
    Article Snippet: Human protein chip assay screening for Aβ-binding proteins A human proteome chip (HuProtTM v3.1, CDI Labs), printed on 75 mm × 25 mm SuperEpoxy 3 glass slides (ArrayIt Corp.) and containing over 20,000 purified full-length human recombinant proteins, was blocked with 2% BSA in PBST for 2 h at room temperature and incubated with Alexa Fluor 532-labeled Aβ (3 μg) at 4 °C for 8 h. After washing and rinsing, the array was dried and scanned using a microarray laser scanner. .. 6E10 (Biolegend, 803003); Aβ (β-Amyloid Rabbit mAb, ABclonal, A26342 for analyzing tissue samples); Kvβ2 (Origene, TA331456 for immunostaining; Neuromab, 75- 021 for Western blot and co-IP); MAP2 (Biolegend, 822501); Ankyrin G (Santa Cruz Biotechnology, sc-12719); EB1 (Santa Cruz Biotechnology, sc47704; ABclonal, A2614); Kv1.2 (ABclonal, A6295); c-Fos (Abcam, AB190289); NeuN (Merck, MAB377); VGLUT1 (ABclonal, A17188); PSD95 (Cell Signaling Technology, 3409); HA (Santa Cruz Biotechnology, sc7392); His (Cell Signaling Technology, 2365); V5 (Abcam, ab27671); FLAG (Sigma, F1804); RFP (MBL, PM005); GFP (Santa Cruz Biotechnology, sc9996); α-Tubulin (Santa Cruz Biotechnology, sc-23948); HRP-Streptavidin (Sigma, RABHRP3); normal mouse IgG (Santa Cruz Biotechnology, sc3877) and normal rabbit IgG (Cell Signaling Technology, 2729S) antibodies were used as controls. .. Intracellular proteins with A-scores exceeding 10,000 were then selected for data presentation (GENEON BIOTECH, Daejeon, Republic of Korea) [8].



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    Image Search Results


    RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: Materials Today Bio

    Article Title: An anti-inflammatory neuroenhancer mitigates amyloid-β pathology to improve Alzheimer's disease therapy

    doi: 10.1016/j.mtbio.2026.102874

    Figure Lengend Snippet: RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: Next, the sections were incubated with HRP-conjugated goat anti-mouse/rabbit IgG polymer for 20 min. To detect the synaptic protein, the sections were incubated with the primary antibody against synaptophysin and PSD95, and then incubated with corresponding secondary antibodies conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, USA) or Alexa Fluor 594 (Santa Cruz Biotechnology, USA), respectively.

    Techniques: Injection